Pyrex: Construction, Property, and Uses in Microbiology

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Pyrex: construction, property, and uses in microbiology

Pyrex是由硼硅酸盐构建的玻璃类型的品牌名称。康宁玻璃公司康宁,纽约,开发的pyrex。从化学上讲,正如硼硅酸盐所暗示的那样,这种类型的玻璃由二氧化硅和一种称为硼氧化物的化学物质的至少5%(玻璃中元素的总重量)组成。这些成分的组合和浓度赋予了对温度变化和腐蚀的极大抗性,例如强酸和碱,即硼硅酸盐玻璃制成的任何容器。这种耐用性使Pyrex玻璃器皿在微生物学实验室中非常有用。

康宁公司科学家在1924年开发Pyrex的开发满足了19世纪始于19世纪的高质量科学玻璃器皿的需求。然后,现有的玻璃器皿被实验室化学物质降解,并在暴露于反复的加热和冷却周期时变得脆弱。Pyrex的配方最大程度地减少了材料扩展和收缩的趋势。这保持了测量仪器(例如刻度圆柱体)的准确性,并克服了重复的高压釜时遇到的脆性sterilization实验室玻璃器皿。

Pyrex glassware immediately found acceptance in the microbiology research community. The popularity of the glassware continues today, despite the development of heat and chemical resistant plastic polymers. Glass is still the preferred container for growingbacteria。This is because the glass can be cleaned using harsh chemicals, which will completely remove any organic material that might otherwise adhere to the sides of the vessel. For applications where the chemical composition and concentrations of the medium components are crucial, such organic contaminants must be removed.

Pyrex glassware is also used to manufacture graduated cylinders that are extremely accurate. In some applications, the exact volume of a liquid is important to achieve. This type of glassware is known as volumetric glassware. Plastic still cannot match the accuracy or the unchanging efficiency of volume delivery that is achieved by Pyrex volumetric glassware.

Another application for borosilicate glass is in the measurement of optical density. For this application, typically specially designed vials are filled with the solution or suspension of interest and then placed in the path of a beam of light in a machine known as a分光光度计。可以记录通过样品的光的量,并且可以使用适当的对照,例如,可以使用样品中的细菌数量。塑料材料不适合光密度测量,因为塑料可能是多云。因此,小瓶本身会吸收一些传入的光。但是,可以制作pyrex,以便在光学上透明。甚至已经制作了生长烧瓶,其中所谓的“侧臂”基本上是融合在烧瓶上的试管,可用于直接获得光密度测量值culturefrom the flask.

In the same vein, the use of optically transparent slabs of Pyrex as显微镜幻灯片是微生物实验室中的基本工具。幻灯片的耐热性允许将样品直接在载玻片上加热。这对于诸如分枝杆菌的酸性染色之类的污渍很重要,其中样品的加热对于细菌的准确染色至关重要。同样,至于光密度测量值,细菌样品的光显微镜检查取决于支撑表面的透明度。塑料不是幻灯片的合适支撑材料。

在微生物学实验室中,Pyrex玻璃器皿在蒸馏水所需的管道中至关重要的另一个领域。水的蒸馏是一个需要水沸腾的过程。管道必须具有耐热性。同样,由于管道的物理擦洗是不可行的,因此管道必须承受苛性化学物质在管道内部表面上搜索有机材料的应用。

其他应用th的硼硅酸盐玻璃器皿e microbiology laboratory include nondisposable Petri plates for the use of solid media, centrifuge tubes, titration cylinders, and the stopcocks that control the flow rate.

热和化学耐药性塑料被广泛用于典型的微生物实验室,尤其是用于常规的高批量操作,在这些实验室中,清洁和制备玻璃器皿以重复使用是耗时的,并且容易出错。

However, the accuracy and advantages of Pyrex glassware ensure its continued use in the most modern of microbiology laboratories.

也可以看看微生物学实验室方法;显微镜